Science Quiz / Agars Differential Media

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Can you name the Agars Differential Media?

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H2S presence
K/A
No RBC lysis
Detects lactose fermentation, H2S production, inhibits non-enterics; Lac ferms: yellow/salmon, nonferms: colorless, H2S: black
G
ferments glucose + lactose and/or sucrose
Cultivation of non-fastidious bacteria; growth indicates presence
Cultivation of fastidious bacteria like Neisseria and Haemophilus
Select for fastidious bacteria within a large flora
Detects glucose, lactose, sucrose fermentation; gas and H2S production
Complete RBC lysis
Selects against enterics and G- using antibiotics
hint
A/A
Gas presence
α: partial clearing, green or brownish ring; β: wide zone of clearing; γ: non-hemolytic
non-fermenter of all 3 sugars
K/K
Contains bile salts and crystal violet which selects for gram-negative enterics, differentiates lactose-fermenters from non-fermenters/ red for lactose ferms and white for nonferms
+
Partial RBC lysis
Differentiates lactose fermenters from non-fermenters/ Lac ferms: blue/black and nonferms: colorless/light purple
ferments glucose only
Selects for Staphylococci, which grow at high salt concentrations

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Created Oct 11, 2017ReportNominate
Tags:agar, differential, media

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