Science Quiz / Agars Differential Media

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Can you name the Agars Differential Media?

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Differentiates lactose fermenters from non-fermenters/ Lac ferms: blue/black and nonferms: colorless/light purple
ferments glucose only
α: partial clearing, green or brownish ring; β: wide zone of clearing; γ: non-hemolytic
+
Detects glucose, lactose, sucrose fermentation; gas and H2S production
K/A
Detects lactose fermentation, H2S production, inhibits non-enterics; Lac ferms: yellow/salmon, nonferms: colorless, H2S: black
Contains bile salts and crystal violet which selects for gram-negative enterics, differentiates lactose-fermenters from non-fermenters/ red for lactose ferms and white for nonferms
Selects for Staphylococci, which grow at high salt concentrations
H2S presence
A/A
Cultivation of non-fastidious bacteria; growth indicates presence
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K/K
Select for fastidious bacteria within a large flora
Complete RBC lysis
Gas presence
G
non-fermenter of all 3 sugars
Partial RBC lysis
ferments glucose + lactose and/or sucrose
No RBC lysis
Selects against enterics and G- using antibiotics
Cultivation of fastidious bacteria like Neisseria and Haemophilus

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Created Oct 11, 2017ReportFavoriteNominate
Tags:agar, differential, media

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