Science Quiz / A few of my favorite Enzymes!

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Can you name the Exam 1 Enzymes?

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Enzyme FunctionEnzyme
Found in mitochondria; Facillitates formation of Orotate
Puts initial RNA primer down
Adds ribonucleotides to 3' end of growing RNA
Introduces negative supercoils into prokaryotic DNA
Eukaryotic DNA: mitochondrial polymerase
Binds TATA box and recurits TAFs to form TFIIID
Recombination protein: generation of ssDNA with 3' overhang
Cleaves C-terminal residue in proteins
Cleaves Leu, Ile & Val on amino side
Nonprotein component that are bound but not necessary
Enzymes that phosphorylate proteins; Involved in intracellular signalling
Enzymes with multiple active sites; Have a hill coefficient >1
One of two enzymes involved in LTR retrotransposition that isn't Reverse transcriptase
Part of RNA Polymerase core: Recognizes the regulatory sequence in the promoter of DNA
Catalyzes unwinding of DNA
Class of enzymes involved in formation of covalent bonds between Carbon & Sulfur, Nitrogen or Oxygen
Converts nucleotide diphosphates into deoxyribonucleotides
Eukaryotic DNA: polymerase of lagging strand
Converts Adenosine into Inosine
Prokaryotic DNA: Adds 9000 bp/min/enzyme, replicates most of DNA in e. coli
Introduces swivel points in DNA to relax positive supercoils; Type that is ATP dependent
Nonprotein groups bound to enzyme
Nonprotein component that helps transfer functional groups
Found on CAD; Facilitates formation of carbamoylphosphate
Eukaryotic DNA: polymerase of leading strand
Helicase + ssDNAbp + Primase + Polymerase III Holoenzyme
Seals single strand nicks in DNA
Recognizes specific tRNA and catalyzes attachment of Amino Acid
Converts Hypoxanthine into Xanthine and then into Uric Acid
Competes for active site; Increases Km of enzyme
Enzyme FunctionEnzyme
Class of enzymes that are involved in oxidation/reductiion reactions; Most use NAD, NADP or FAD
Part of RNA Polymerase holoenzyme: Increases affinity of RNA polymerase core for promoter DNA by 10,000x
Found on UMP Synthase; Facilitates formation of UMP
Removes abasic nucleotide
Binds to cyclins to regulate the cell cycle
Cleaves Lys & Arg on their carboxyl side
Cleaves phosphate backbone of DNA
Mediates CSSR
Class of enzymes involved in nonhydrolytic cleavages between carbon & N,C or S
Prokaryotic DNA: Removes RNA primer and involved in DNA repair in Prokaryotes
Hydrolyzes sucrose to glucose and fructose
Introduces double strand breaks in meiotic DNA cells
Removes damaged DNA bases
Reacts with all Amino Acids except for the C-terminal
Found on CAD; Facilitates formation of carbamoylaspartate
Assembles from U1-U6 proteins & snRNA; Involved in splicing
Eukaryotic RNA polymerase: Transcribes all protein coding genes & SnoRNA, miRNA, SiRNA, and most SnRNA
Eukaryotic DNA: Similary to polymerase I, functions in repair
Involved in transfer of glycan to asparagine residues
Initiaties Nucleophilic attack on A residue within intron initiates cut at 5' splice site
Hydrolyzes alpha-1,4 glycosidic bonds in starch and glycogen in the stomach
Involved in recombination: Used for DNA transposition
Involved in synthesis of TMP
Same enzymes formula, but different structural arrangements
Part of RNA Polymerase core: Holds on to the DNA template
One of two enzymes involved in Poly-A retrotransposition that isn't reverse transcriptase
Found on UMP Synthase; Facillitates formation of OMP
Modifies Histone tail by removing acetly group
Recombination protein: makes nicks for resolution
Add sugars to membrane bound lipid, Dolicol-phosphate
Enzyme FunctionEnzyme
Prokaryotic DNA: Involved in DNA repair, lacks proofreading
Act as CI with PABA to prevent folic acid production in bacteria
Hydrolyzes lactose to glucose and galactose
One of two enzymes involved in purine salvage pathway
Recombination protein: branch migration
Forms ESI complex; lowers Vmax of enzyme
Eukaryotic RNA polymerase: Transcribes tRNA, some rRNA & some snRNA
Adds nucleotides to growing DNA strand; 3'-5' exonuclease activity, 5'-3' exonuclease activity
Reverse transcriptase; Adds repeating units to 5' end of DNA
Other of two enzymes involved in purine salvage pathway
Class of enzymes involved in hydrolytic cleavage of covalent bonds between carbon and another atom
Modifies Histone tail by adding acetly group
Recombination protein: stabilization of ssDNA, assembles Holliday junction
Eukaryotic RNA polymerase: Transcribes 5.8S, 18S & 28S rRNA genes
Hydrolyzes alpha-limit dextrins
Hydrolyzes disaccharides and products of amylases; Brush border enzymes
Hydrolyzes alpha-1,4 glycosidic bonds in starch and glycogen in the mouth
Class of enzymes involved in molecular rearrangement in a molecule
Cleaves Tyr, Trp & Phe on their carboxyl side
Found on CAD; Facilitates formation of Dihyrdroorate
Eukaryotic DNA: tightly interacts with primase
Class of enzymes involved in transfer of a chemical group from a donor to an acceptor
Introduces swivel points in DNA to relax positive supercoils; Type that is ATP Independent
Availability of these can determines activation of retinoblastoma protein
Prokaryotic DNA: No proofreading activity, DNA repair
Binds single stranded DNA tightly and prevents degradation
Hydrolyzes maltose and maltotriose to glucose
Enzyme involved in reaction of PRPP and Glutamine to 5-phophoribosylamine and glutamate
Part of RNA Polymerase core: Catalyzes phosphodiester backbone formation in 5'-->3' direction

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