Science Quiz / A few of my favorite Enzymes!

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Can you name the Exam 1 Enzymes?

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Enzyme FunctionEnzyme
Mediates CSSR
Adds ribonucleotides to 3' end of growing RNA
Class of enzymes involved in transfer of a chemical group from a donor to an acceptor
Introduces double strand breaks in meiotic DNA cells
Cleaves Tyr, Trp & Phe on their carboxyl side
Same enzymes formula, but different structural arrangements
Reverse transcriptase; Adds repeating units to 5' end of DNA
Part of RNA Polymerase core: Recognizes the regulatory sequence in the promoter of DNA
Catalyzes unwinding of DNA
Involved in synthesis of TMP
Binds TATA box and recurits TAFs to form TFIIID
Other of two enzymes involved in purine salvage pathway
Adds nucleotides to growing DNA strand; 3'-5' exonuclease activity, 5'-3' exonuclease activity
Add sugars to membrane bound lipid, Dolicol-phosphate
Converts Hypoxanthine into Xanthine and then into Uric Acid
Assembles from U1-U6 proteins & snRNA; Involved in splicing
Class of enzymes involved in hydrolytic cleavage of covalent bonds between carbon and another atom
Seals single strand nicks in DNA
Removes abasic nucleotide
Binds single stranded DNA tightly and prevents degradation
Introduces swivel points in DNA to relax positive supercoils; Type that is ATP dependent
Found on UMP Synthase; Facillitates formation of OMP
Hydrolyzes alpha-1,4 glycosidic bonds in starch and glycogen in the mouth
Prokaryotic DNA: Adds 9000 bp/min/enzyme, replicates most of DNA in e. coli
Found in mitochondria; Facillitates formation of Orotate
Nonprotein component that are bound but not necessary
Eukaryotic DNA: Similary to polymerase I, functions in repair
Part of RNA Polymerase core: Holds on to the DNA template
Prokaryotic DNA: No proofreading activity, DNA repair
Part of RNA Polymerase holoenzyme: Increases affinity of RNA polymerase core for promoter DNA by 10,000x
Enzyme FunctionEnzyme
Class of enzymes that are involved in oxidation/reductiion reactions; Most use NAD, NADP or FAD
Recombination protein: branch migration
Converts Adenosine into Inosine
Eukaryotic DNA: polymerase of leading strand
Prokaryotic DNA: Removes RNA primer and involved in DNA repair in Prokaryotes
Forms ESI complex; lowers Vmax of enzyme
Cleaves phosphate backbone of DNA
Introduces swivel points in DNA to relax positive supercoils; Type that is ATP Independent
Modifies Histone tail by adding acetly group
Class of enzymes involved in formation of covalent bonds between Carbon & Sulfur, Nitrogen or Oxygen
Converts nucleotide diphosphates into deoxyribonucleotides
Cleaves Leu, Ile & Val on amino side
Involved in recombination: Used for DNA transposition
Hydrolyzes alpha-limit dextrins
One of two enzymes involved in purine salvage pathway
One of two enzymes involved in LTR retrotransposition that isn't Reverse transcriptase
Reacts with all Amino Acids except for the C-terminal
Puts initial RNA primer down
Part of RNA Polymerase core: Catalyzes phosphodiester backbone formation in 5'-->3' direction
Found on UMP Synthase; Facilitates formation of UMP
Recombination protein: generation of ssDNA with 3' overhang
Competes for active site; Increases Km of enzyme
Initiaties Nucleophilic attack on A residue within intron initiates cut at 5' splice site
Hydrolyzes disaccharides and products of amylases; Brush border enzymes
Cleaves Lys & Arg on their carboxyl side
Introduces negative supercoils into prokaryotic DNA
Class of enzymes involved in molecular rearrangement in a molecule
Nonprotein component that helps transfer functional groups
Hydrolyzes sucrose to glucose and fructose
One of two enzymes involved in Poly-A retrotransposition that isn't reverse transcriptase
Enzyme FunctionEnzyme
Cleaves C-terminal residue in proteins
Recombination protein: makes nicks for resolution
Class of enzymes involved in nonhydrolytic cleavages between carbon & N,C or S
Eukaryotic RNA polymerase: Transcribes all protein coding genes & SnoRNA, miRNA, SiRNA, and most SnRNA
Eukaryotic DNA: mitochondrial polymerase
Hydrolyzes lactose to glucose and galactose
Enzymes that phosphorylate proteins; Involved in intracellular signalling
Found on CAD; Facilitates formation of carbamoylaspartate
Eukaryotic RNA polymerase: Transcribes 5.8S, 18S & 28S rRNA genes
Eukaryotic DNA: polymerase of lagging strand
Found on CAD; Facilitates formation of Dihyrdroorate
Hydrolyzes maltose and maltotriose to glucose
Binds to cyclins to regulate the cell cycle
Prokaryotic DNA: Involved in DNA repair, lacks proofreading
Modifies Histone tail by removing acetly group
Enzyme involved in reaction of PRPP and Glutamine to 5-phophoribosylamine and glutamate
Eukaryotic RNA polymerase: Transcribes tRNA, some rRNA & some snRNA
Act as CI with PABA to prevent folic acid production in bacteria
Nonprotein groups bound to enzyme
Found on CAD; Facilitates formation of carbamoylphosphate
Recognizes specific tRNA and catalyzes attachment of Amino Acid
Eukaryotic DNA: tightly interacts with primase
Involved in transfer of glycan to asparagine residues
Removes damaged DNA bases
Hydrolyzes alpha-1,4 glycosidic bonds in starch and glycogen in the stomach
Recombination protein: stabilization of ssDNA, assembles Holliday junction
Availability of these can determines activation of retinoblastoma protein
Helicase + ssDNAbp + Primase + Polymerase III Holoenzyme
Enzymes with multiple active sites; Have a hill coefficient >1

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Created Aug 21, 2010ReportNominate

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