Science Quiz / A few of my favorite Enzymes!

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Can you name the Exam 1 Enzymes?

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Enzyme FunctionEnzyme
Hydrolyzes lactose to glucose and galactose
Act as CI with PABA to prevent folic acid production in bacteria
Class of enzymes involved in nonhydrolytic cleavages between carbon & N,C or S
Part of RNA Polymerase holoenzyme: Increases affinity of RNA polymerase core for promoter DNA by 10,000x
Involved in transfer of glycan to asparagine residues
Found in mitochondria; Facillitates formation of Orotate
Availability of these can determines activation of retinoblastoma protein
Hydrolyzes alpha-1,4 glycosidic bonds in starch and glycogen in the stomach
Nonprotein component that helps transfer functional groups
Cleaves Leu, Ile & Val on amino side
Converts nucleotide diphosphates into deoxyribonucleotides
Eukaryotic RNA polymerase: Transcribes all protein coding genes & SnoRNA, miRNA, SiRNA, and most SnRNA
Seals single strand nicks in DNA
Eukaryotic RNA polymerase: Transcribes tRNA, some rRNA & some snRNA
Eukaryotic DNA: mitochondrial polymerase
One of two enzymes involved in LTR retrotransposition that isn't Reverse transcriptase
Prokaryotic DNA: No proofreading activity, DNA repair
Cleaves Lys & Arg on their carboxyl side
Eukaryotic RNA polymerase: Transcribes 5.8S, 18S & 28S rRNA genes
Enzymes with multiple active sites; Have a hill coefficient >1
Eukaryotic DNA: Similary to polymerase I, functions in repair
Prokaryotic DNA: Adds 9000 bp/min/enzyme, replicates most of DNA in e. coli
Recognizes specific tRNA and catalyzes attachment of Amino Acid
Catalyzes unwinding of DNA
Class of enzymes involved in transfer of a chemical group from a donor to an acceptor
Recombination protein: makes nicks for resolution
Enzyme involved in reaction of PRPP and Glutamine to 5-phophoribosylamine and glutamate
Recombination protein: stabilization of ssDNA, assembles Holliday junction
Converts Hypoxanthine into Xanthine and then into Uric Acid
Modifies Histone tail by adding acetly group
Enzyme FunctionEnzyme
Binds single stranded DNA tightly and prevents degradation
Nonprotein groups bound to enzyme
Recombination protein: generation of ssDNA with 3' overhang
Same enzymes formula, but different structural arrangements
Mediates CSSR
Adds nucleotides to growing DNA strand; 3'-5' exonuclease activity, 5'-3' exonuclease activity
Recombination protein: branch migration
Converts Adenosine into Inosine
Part of RNA Polymerase core: Holds on to the DNA template
Eukaryotic DNA: polymerase of lagging strand
Introduces negative supercoils into prokaryotic DNA
Hydrolyzes alpha-1,4 glycosidic bonds in starch and glycogen in the mouth
Assembles from U1-U6 proteins & snRNA; Involved in splicing
Nonprotein component that are bound but not necessary
Involved in recombination: Used for DNA transposition
Found on CAD; Facilitates formation of carbamoylaspartate
Hydrolyzes maltose and maltotriose to glucose
Other of two enzymes involved in purine salvage pathway
Class of enzymes that are involved in oxidation/reductiion reactions; Most use NAD, NADP or FAD
Reacts with all Amino Acids except for the C-terminal
Binds TATA box and recurits TAFs to form TFIIID
One of two enzymes involved in Poly-A retrotransposition that isn't reverse transcriptase
Found on CAD; Facilitates formation of carbamoylphosphate
Class of enzymes involved in hydrolytic cleavage of covalent bonds between carbon and another atom
Add sugars to membrane bound lipid, Dolicol-phosphate
Introduces swivel points in DNA to relax positive supercoils; Type that is ATP dependent
Hydrolyzes alpha-limit dextrins
Prokaryotic DNA: Removes RNA primer and involved in DNA repair in Prokaryotes
Class of enzymes involved in formation of covalent bonds between Carbon & Sulfur, Nitrogen or Oxygen
Class of enzymes involved in molecular rearrangement in a molecule
Enzyme FunctionEnzyme
Cleaves C-terminal residue in proteins
Found on UMP Synthase; Facillitates formation of OMP
Reverse transcriptase; Adds repeating units to 5' end of DNA
One of two enzymes involved in purine salvage pathway
Eukaryotic DNA: tightly interacts with primase
Cleaves Tyr, Trp & Phe on their carboxyl side
Competes for active site; Increases Km of enzyme
Hydrolyzes sucrose to glucose and fructose
Introduces swivel points in DNA to relax positive supercoils; Type that is ATP Independent
Forms ESI complex; lowers Vmax of enzyme
Found on UMP Synthase; Facilitates formation of UMP
Hydrolyzes disaccharides and products of amylases; Brush border enzymes
Introduces double strand breaks in meiotic DNA cells
Removes abasic nucleotide
Helicase + ssDNAbp + Primase + Polymerase III Holoenzyme
Cleaves phosphate backbone of DNA
Puts initial RNA primer down
Involved in synthesis of TMP
Part of RNA Polymerase core: Recognizes the regulatory sequence in the promoter of DNA
Removes damaged DNA bases
Binds to cyclins to regulate the cell cycle
Modifies Histone tail by removing acetly group
Adds ribonucleotides to 3' end of growing RNA
Initiaties Nucleophilic attack on A residue within intron initiates cut at 5' splice site
Prokaryotic DNA: Involved in DNA repair, lacks proofreading
Found on CAD; Facilitates formation of Dihyrdroorate
Eukaryotic DNA: polymerase of leading strand
Enzymes that phosphorylate proteins; Involved in intracellular signalling
Part of RNA Polymerase core: Catalyzes phosphodiester backbone formation in 5'-->3' direction

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Created Aug 21, 2010ReportNominate

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