Science Quiz / A few of my favorite Enzymes!

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Can you name the Exam 1 Enzymes?

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Enzyme FunctionEnzyme
Eukaryotic DNA: Similary to polymerase I, functions in repair
Helicase + ssDNAbp + Primase + Polymerase III Holoenzyme
Other of two enzymes involved in purine salvage pathway
Nonprotein component that helps transfer functional groups
Introduces swivel points in DNA to relax positive supercoils; Type that is ATP dependent
Modifies Histone tail by adding acetly group
Reacts with all Amino Acids except for the C-terminal
Eukaryotic RNA polymerase: Transcribes all protein coding genes & SnoRNA, miRNA, SiRNA, and most SnRNA
Removes damaged DNA bases
Found on UMP Synthase; Facilitates formation of UMP
Enzyme involved in reaction of PRPP and Glutamine to 5-phophoribosylamine and glutamate
Hydrolyzes sucrose to glucose and fructose
Found in mitochondria; Facillitates formation of Orotate
Involved in synthesis of TMP
Eukaryotic DNA: tightly interacts with primase
Competes for active site; Increases Km of enzyme
Prokaryotic DNA: Removes RNA primer and involved in DNA repair in Prokaryotes
Adds ribonucleotides to 3' end of growing RNA
Class of enzymes involved in formation of covalent bonds between Carbon & Sulfur, Nitrogen or Oxygen
Recognizes specific tRNA and catalyzes attachment of Amino Acid
Recombination protein: branch migration
Binds to cyclins to regulate the cell cycle
Nonprotein groups bound to enzyme
Recombination protein: stabilization of ssDNA, assembles Holliday junction
Hydrolyzes lactose to glucose and galactose
Eukaryotic DNA: polymerase of leading strand
Puts initial RNA primer down
Modifies Histone tail by removing acetly group
Cleaves Tyr, Trp & Phe on their carboxyl side
Reverse transcriptase; Adds repeating units to 5' end of DNA
Enzyme FunctionEnzyme
Part of RNA Polymerase core: Recognizes the regulatory sequence in the promoter of DNA
Forms ESI complex; lowers Vmax of enzyme
Introduces swivel points in DNA to relax positive supercoils; Type that is ATP Independent
Eukaryotic RNA polymerase: Transcribes 5.8S, 18S & 28S rRNA genes
One of two enzymes involved in LTR retrotransposition that isn't Reverse transcriptase
Assembles from U1-U6 proteins & snRNA; Involved in splicing
One of two enzymes involved in Poly-A retrotransposition that isn't reverse transcriptase
Prokaryotic DNA: Adds 9000 bp/min/enzyme, replicates most of DNA in e. coli
Part of RNA Polymerase core: Holds on to the DNA template
Adds nucleotides to growing DNA strand; 3'-5' exonuclease activity, 5'-3' exonuclease activity
Class of enzymes involved in transfer of a chemical group from a donor to an acceptor
Hydrolyzes alpha-1,4 glycosidic bonds in starch and glycogen in the stomach
Removes abasic nucleotide
Found on CAD; Facilitates formation of carbamoylaspartate
Part of RNA Polymerase holoenzyme: Increases affinity of RNA polymerase core for promoter DNA by 10,000x
Cleaves C-terminal residue in proteins
Act as CI with PABA to prevent folic acid production in bacteria
Availability of these can determines activation of retinoblastoma protein
Binds single stranded DNA tightly and prevents degradation
Involved in transfer of glycan to asparagine residues
Prokaryotic DNA: Involved in DNA repair, lacks proofreading
Seals single strand nicks in DNA
Class of enzymes involved in nonhydrolytic cleavages between carbon & N,C or S
Same enzymes formula, but different structural arrangements
Introduces negative supercoils into prokaryotic DNA
Recombination protein: makes nicks for resolution
Cleaves phosphate backbone of DNA
Eukaryotic DNA: polymerase of lagging strand
Recombination protein: generation of ssDNA with 3' overhang
Found on CAD; Facilitates formation of Dihyrdroorate
Enzyme FunctionEnzyme
Class of enzymes that are involved in oxidation/reductiion reactions; Most use NAD, NADP or FAD
Converts nucleotide diphosphates into deoxyribonucleotides
One of two enzymes involved in purine salvage pathway
Cleaves Leu, Ile & Val on amino side
Binds TATA box and recurits TAFs to form TFIIID
Catalyzes unwinding of DNA
Mediates CSSR
Enzymes with multiple active sites; Have a hill coefficient >1
Class of enzymes involved in hydrolytic cleavage of covalent bonds between carbon and another atom
Part of RNA Polymerase core: Catalyzes phosphodiester backbone formation in 5'-->3' direction
Prokaryotic DNA: No proofreading activity, DNA repair
Eukaryotic RNA polymerase: Transcribes tRNA, some rRNA & some snRNA
Hydrolyzes disaccharides and products of amylases; Brush border enzymes
Enzymes that phosphorylate proteins; Involved in intracellular signalling
Found on CAD; Facilitates formation of carbamoylphosphate
Converts Hypoxanthine into Xanthine and then into Uric Acid
Cleaves Lys & Arg on their carboxyl side
Involved in recombination: Used for DNA transposition
Nonprotein component that are bound but not necessary
Introduces double strand breaks in meiotic DNA cells
Add sugars to membrane bound lipid, Dolicol-phosphate
Class of enzymes involved in molecular rearrangement in a molecule
Hydrolyzes alpha-limit dextrins
Eukaryotic DNA: mitochondrial polymerase
Initiaties Nucleophilic attack on A residue within intron initiates cut at 5' splice site
Hydrolyzes alpha-1,4 glycosidic bonds in starch and glycogen in the mouth
Hydrolyzes maltose and maltotriose to glucose
Found on UMP Synthase; Facillitates formation of OMP
Converts Adenosine into Inosine

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