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Techniques in Molecular Biology

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HintAnswer
Phase containing RNA after trizol purification.
Denatures proteins and provides them with a negative charge.
A powerful protein denaturant used in RNA isolation to lyse cells and denature RNases
The pH of the separating gel
Catalyses polymerisation of acrylamide gel.
Alkylates thiol groups on proteins preventing reoxidation during gel electrophoresis.
What does the lac I gene encode?
Lacotse analogue not metabolised by bacteria
HintAnswer
Denatures and solubilizes proteins for gel electrophoresis.
Which type of gel is used in 2D gel electrophoresis
Molecule used to elute proteins with a poly histidine tag bound in affinity chromatography.
In a discontinuous gel, which gel has larger pores?
Site in a plasmid containing many restriction enzyme sites.
Substance used to precipitate RNA
The pH of the stacking gel
Activates acrylamide monomers by forming free radicals when dissolved in water.

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