Science Quiz / Techniques in Molecular Biology

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Techniques in Molecular Biology

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HintAnswer
Phase containing RNA after trizol purification.
The pH of the separating gel
Activates acrylamide monomers by forming free radicals when dissolved in water.
Denatures and solubilizes proteins for gel electrophoresis.
Substance used to precipitate RNA
Lacotse analogue not metabolised by bacteria
Alkylates thiol groups on proteins preventing reoxidation during gel electrophoresis.
The pH of the stacking gel
HintAnswer
In a discontinuous gel, which gel has larger pores?
A powerful protein denaturant used in RNA isolation to lyse cells and denature RNases
Site in a plasmid containing many restriction enzyme sites.
Which type of gel is used in 2D gel electrophoresis
Molecule used to elute proteins with a poly histidine tag bound in affinity chromatography.
Denatures proteins and provides them with a negative charge.
Catalyses polymerisation of acrylamide gel.
What does the lac I gene encode?

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