Science Quiz / Techniques in Molecular Biology

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Techniques in Molecular Biology

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Also try: Calculus A-Z
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Denatures and solubilizes proteins for gel electrophoresis.
What does the lac I gene encode?
Denatures proteins and provides them with a negative charge.
Which type of gel is used in 2D gel electrophoresis
Phase containing RNA after trizol purification.
A powerful protein denaturant used in RNA isolation to lyse cells and denature RNases
The pH of the separating gel
The pH of the stacking gel
Lacotse analogue not metabolised by bacteria
In a discontinuous gel, which gel has larger pores?
Molecule used to elute proteins with a poly histidine tag bound in affinity chromatography.
Site in a plasmid containing many restriction enzyme sites.
Substance used to precipitate RNA
Catalyses polymerisation of acrylamide gel.
Alkylates thiol groups on proteins preventing reoxidation during gel electrophoresis.
Activates acrylamide monomers by forming free radicals when dissolved in water.

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