| Question | Answer |
| What is Dr. Horrible's real name? | |
| Who collected Dr. Horrible's mail for him? | |
| What organization does Dr. Horrible want to join? | |
| Who is the leader of the ELE? | |
| Where did Dr. Horrible go to to see Penny on a regular basis? | |
| What did Dr. Horrible want to steal? | |
| How was it being transported? | |
| What did he need it for? | |
| Whose hair was 'blowing in the breeze'? | |
| What does Dr. Horrible sneak behind when spying on Captain Hammer and Penny? | |
| 'I wanna be an achiever...like Bad Horse.' 'The Thoroughbred of Sin?' 'I mean ________.' | |
| What dessert does Dr. Horrible get for him and Penny? | |
| What do they eat it with? | |
| 'You don't happen to like frozen yogurt do you?' 'I love it!' 'What a _____________.' | |
| What organization does Penny volunteer for? | |
| How many groupies are there? | |
| What does Dr. Horrible plan to use on Captain Hammer? | |
| What's so special about Captain Hammer's deltoids? | |
| Who dies as a result of the broken Death Ray? | |
| What does Moist drop repeatedly on a heist with Dr. Horrible? | |