| Rank/Character/Film | Actor |
| 26. Stoker Thompson (The Set-Up) | |
| 27. Augustus Pinkerton (The Big Street) | |
| 28. Brick Pollitt (Cat on a Hot Tin Roof) | |
| 29. Dr. Mabuse (Dr. Mabuse, the Gambler) | |
| 30. Eric Stanton (Fallen Angel) | |
| 31. Johnny Clay (The Killing) | |
| 32. Bruno Anthony (Strangers on a Train) | |
| 33. J.J. Hunsecker (Sweet Smell of Success) | |
| 34. Hank Quinlan (Touch of Evil) | |
| 35. Joe Adams/Joe Bascopolous (Mr. Lucky) | |
| 36. Captain Ralls (Wake of the Red Witch) | |
| 37. Dr. Samuel A. Mudd (The Prisoner of Shark Island) | |
| 38. Pike Bishop (The Wild Bunch) | |
| 39. John Robie (To Catch a Thief) | |
| 40. Roy Earle (High Sierra) | |
| 41. John Russell (Hombre) | |
| 42. Scottie Ferguson (Vertigo) | |
| 43. Charles Foster Kane (Citizen Kane) | |
| 44. Joe Sullivan (Raw Deal) | |
| 45. Griff Bonnell (Forty Guns) | |
| 46. Ben Wade (3:10 to Yuma) | |
| 47. O'Hara (The General Died at Dawn) | |
| 48. Nick Charles (The Thin Man) | |
| 49. Legionnaire Tom Brown (Morocco) | |
| 50. Philip Raven (This Gun for Hire) | |