| Question | Answer |
| What is the building that the courier van is parked in front of? | |
| What substance does Dr. Horrible need to obtain to create his freeze ray? | |
| What should one not do to this substance? | |
| What is the last word in the first act? | |
| What does Moist's shirt say as he discusses Dr. Horrible's plan to get into the ELE by assassination? | |
| Captain Hammer doesn't go to the gym, because he's just | |
| What country does Dr. Horrible plan to give to Penny? | |
| As Dr. Horrible sits in his armchair, it can be seen that he has two copies of the same book. What is this book? | |
| The actors who play groupies number 1, 2, and 3, respectively: | |
| ELE members: Professor ________ | |