| Step | Enzyme |
| step 1: glucose to glucose-6-phosphate | |
| step 2: glucose-6-phosphate to fructose-6-phosphate | |
| step 3: fructose-6-phosphate to fructose-1,6-biphosphate | |
| step 4: fructose-1,6- biphosphate to glyceraldehyde-3-phosphate or dihydroxyacetone phosphate | |
| step 5:glyceraldehyde-3-phosphate to dihydroxyacetone phosphate (and vice versa) | |
| step 6:glyceraldehyde-3-phosphate or dihydroxyacetone phosphate to (2) 1,3-bisphosphoglycerate | |
| step 7: (2) 1,3-bisphosphoglycerate to (2) 3-phosphoglycerate | |
| step 8: (2) 3-phosphoglycerate to (2) 2-phosphoglycerate | |
| step 9:(2) 2-phosphoglycerate to (2) phosphoenolpyruvate | |
| step 10:(2) phosphoenolpyruvate to (2) pyruvate | |