| Clue | Villain |
| Spidey's main villain. Okay, can't go wrong here. | |
| A classic villain who first appears in Amazing Spider-Man #9 | |
| A classic reptilian villain from ASM 6 | |
| Another classic originally from ASM 15 | |
| An obscure villain from the nineties, son of previous | |
| A popular serial killer villain introduced in the late 90's | |
| This villain is made out of bees | |
| An original villain created for the musical (just type 'Swiss Miss') | |
| Some weird original character named Arachne is in the musical but I can't tell if it's a villain or not, so you get bonus answer credit | |
| Nope, Doc Ock would have been the perfect villain for a stage production and thus was not selected. | |
| 'A classic villain that would be easy to pull off on stage? Pass, we need more men made out of bees.' | |
| No, he was too busy trying to save his daughter and floating away aimlessly. | |
| Not suitable for a family musical, his name has too many unfortunate implications | |
| That would have been great, but only if he was voiced by Mark Hamill again. | |
| Not on the roster, but putting in his offspring without him makes sense for some reason | |
| OK, he's in the musical and is kind of an antagonist. Partial credit for you! | |
| The most horrible villains of them all. | |
| Clearly some kind of diabolical mastermind. Or a complete idiot, either way. | |