| Role | Name | Lifetime |
| Founder of the Medici bank | |
| 'Pater Patriae' | |
| Founder of the Popolani branch of the family | |
| 'The Gouty' | |
| Patron of Donatello | |
| 'The Magnificent' | |
| Murdered in the Cathedral | |
| One of his assassins, from a rival family | |
| His other assassin | |
| 'The Unfortunate' | |
| Wife of Jacopo Salviati | |
| Monk who drove the Medici out of Florence | |
| 'The Popular' | |
| His brother, also known as 'The Popular' | |
| First Medici Pope | |
| Duke of Nemours | |
| Second Medici Pope | |
| Duke of Urbino | |
| The last of the great condottieri | |
| | Role | Name | Lifetime |
| Illegitimate son of the Duke of Nemours | |
| 'Il Moro', first Duke of Florence | |
| Assassinated the Duke, his cousin | |
| 1st Grand Duke of Tuscany | |
| Queen of France | |
| Her son, King of France | |
| Her son, King of France | |
| Her son, King of France | |
| Third Medici Pope | |
| 2nd Grand Duke of Tuscany | |
| 3rd Grand Duke of Tuscany | |
| Commander-in-Chief of the army of Venice | |
| Queen of France | |
| Her son, King of France | |
| 4th Grand Duke of Tuscany | |
| Cardinal-Bishop of Ostia | |
| 5th Grand Duke of Tuscany | |
| 6th Grand Duke of Tuscany | |
| 7th Grand Duke of Tuscany | |
|