| Description | Character |
| Protagonist of the first three games. Known for his famous 'Objection!' | |
| His excitable spirit medium partner. Loves hamburgers | |
| His mentor. Occasionally gives advice via the above character's body. | |
| Main antagonist. Also protagonist's childhood friend. | |
| Ruthless prosecutor. Known for his nearly perfect record. | |
| His daughter. Is a child prodigy. | |
| Chief of police. Designed the police mascot. | |
| The previously mentioned police mascot. | |
| Hapless detective. Usually works for the prosecutor, but tends to help you anyway. | |
| Mysterious coffee-addicted prosecutor. | |
| 16-year-old forensic investigator. | |
| And her sister, the former Chief Prosecutor. | |
| Furious loan shark. Polar opposite of the protagonist. | |
| A frequent client in the games. Dubbed the 'Goddess of Misfortune.' | |
| Master spirit medium at the age of 8. | |
| An 'innocent' young woman. Has a fondness for poison. | |
| Hot-headed photographer from the 'heartland.' | |