| Origin | Villain | First Appearance |
| Carves his alias out of his father's coffin due to family complications and personal failures | |
| Several versions, usually unfortunate incidents involving genetic changes mold this villain | |
| Insane psychiatrist whose best weapon is Batman's identity | |
| Chemical encounter and an undying passion to find a cure for his icy wife | |
| Fabled Lazarus pits grant this assassin immortality | |
| Batty transformation after using a solution to prevent his deafness | |
| Society outcast for bird-like appearance | |
| History teacher with too much enthusiasm for Greek Mythology | |
| Obsessions with puzzles gives him a questionable drive | |
| Serving his father's life sentence, prison and Venom fuel this villain | |
| Left for dead in an Arkham freezer, frostbite deforms his face and drives him mad | |