DNA Replication, Repair, & Recombination

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Can you name the relevant terms of DNA replication, repair, & recombination?

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ClueAnswer
Associated with each core polymerase domain is a ______, which holds the DNA in place.
These proteins are the first to bind to the bacterial chromosome in replication, and do so upstream of the DUE.
______ excision repair is employed when a mismatched base must be removed, followed by the rest of the nucleotide.
This enzyme is coupled to polymerase, and unwinds the DNA at the replication fork.
DNA replication is semiconservative, meaning that each daughter molecule has ___ original strand and ___ new strand.
Because DNA polymerase can only polymerize existing nucleic acids, it requires an RNA _____ to begin elongation.
This is the most common nitrogenous base to dimerize in response to UV light exposure.
This process proceeds DNA replication at eukaryotic origins.
Just type in 'heteroduplex'. I'm all quizzed out.
Another form of DNA damage due to hydrolytic attack, where cytosine is converted to uracil.
The number of nucleotides processed by DNA polymerase before its dissociation from DNA is referred to as the polymerase's:
This protein initiates recombination in meiosis.
These proteins continually bind to isolated DNA strands during replication, in order to stabilize and protect them.
This is the most common cause of point deletions, due to hydrolytic attack between the nitrogenous base and sugar.
This enzyme undoes coils formed upstream of the replication fork due to supercoiling.
True or false: there is a biological advantage to limited mutation.
This is formed during recombination of sister chromatids; its resolution determines the ultimate resulting sequences.
These 'chunks' of DNA, produced during DNA elongation, are the only way to synthesize off the lagging strand, due to the unidirectionality of DNA polymerase.
ClueAnswer
Immediately following DNA replication in the bacterium, the daughter chromosomes are catenated. What TYPE of topoisomerase is required to separate them?
The DUE is rich in the nitrogenous bases ________ and _______, enabling its rapid denaturation.
The enyzme _________ uses an internal RNA template to extend the ends of chromosomes as a safeguard against degradation, leaving a _' overhang that is tucked into the molecule.
The helicase involved in DNA replication in eukaryotes is also known as this.
This type of recombination is limited to particular areas of the chromosome.
The energy required for elongation in DNA synthesis is provided by the release of _________ from nucleotides.
The origin of replication for the bacterial chromosome is also known as:
_______ excision repair depends on removing an entire segment of DNA containing the defect, such as dimerized bases.
The protein complex that assembles at eukaryotic origins of replication:
These proteins are present ONLY during S phase, due to the cell cycle's regulation, and are essential for loading helicase onto DNA.
True or false: while there are multiple origins of replication in the eukaryotic genome, there is still only one per chromosome.
Replication termination is signaled by ___ proteins binding to ___ sequences, causing the arrest of replication forks.
Nonhomologous end joining as a form of double-stranded break repair is mediated by ____ proteins, but ultimately leads to the accrual of permanent DNA deletions called _____.
This form of double-stranded break repair depends on recombination with sister chromatids for more effective repair, and is thus limited to the S and G2 phases of the cell cycle.
What two molecules can be DIRECTLY synthesized using DNA as a template?
The exonuclease activity of DNApol II proceeds in the _'-to-_' direction.
This segment of DNA at the origin of replication is where the strands first denature in replication.

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