| Character | Answer |
| Thane's son and another of the few Drell encountered. | |
| Encountered on Horizon if Ashley was killed on Virmire. | |
| The self-proclaimed queen of Omega. | |
| Salarian scientist that participated in the modification of the Genophage. | |
| Tali's father. | |
| Probably the most skilled assassin in the galaxy and one of the few Drell encountered in the game. | |
| The galaxy's most enigmatic thief, available from DLC. | |
| Genetically engineered Krogan supersoldier. | |
| The AI of the Normandy. | |
| Main Character | |
| The leader of the Blue Suns encountered on Zaeed's loyalty mission. | |
| A female engineer on the Normandy. | |
| Turian rebel met in the original Mass Effect. | |
| Asari squad member from the original Mass Effect, encountered on Illium. | |
| Quarian marine of the Migrant Fleet encountered on Tali's recruitment mission. | |
| Met in the first Mass Effect, she is a known Quarian engineer. | |
| Batarian sergeant determined to kill Archangel. | |
| Feared bounty hunter available from DLC. | |
| Cerberus officer, genetically modified. | |
| Encountered on Horizon if Kaidan was killed on Virmire. | |