MCDB 205 - Cell Components and Research

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DefinitionVocab Word
Proteins that actively transmit materials through the plasma membrane
A type of method of preparation that embeds cells in plastic and then slices it
Polypeptide chain of amino acids, many uses
Flattened bags of membrane with associated ribosomes that create integral proteins
A protein that glows green and allows labling of cellular components
Lipid Bilayer that surrounds the cell and has protiens and transmitters
'microfilaments,' tracks for myosin motors
The copying of DNA into mRNA strands in nucleus
Lens that collects and recombines beams of light
A method of microscopy that just shines light and captures visible light on a white background
The DNA partner of a strand of RNA
A type of microscopy that uses the absorbance and emission of light
A method that seperates components by interactions
Fusions with this enzyme are used for chromatography because it binds well to glutathione
Small organelles formed from microtubules that extend outside the cell, provide sensory information and motion
A reaction to produce large amounts of DNA segments using restriction enzymes
A method where the interaction of two proteins attached to polymerase allow synthesis of a protein
Dictyostelium Discoideum - used to study basic cell functions
A cylindrical polymer of tubulin that forms cilia, flagella and mitotic spindles, provides tracks for dynein and kinesin
The set of all genes in an organism
A sequence of DNA or RNA that codes for a protein
Organisms that have had so much study done on them that they are easy to use
Usually single stranded molecule composed of phosphate, sugar, base
Large gated channels that control all traffic to the nucleus
C. Elegans - used to study animal functions
Drosophila Melanogaster - used to study multicellular functions
The formation of cellular components from molecules without aid of templates or enzymes
Starting with a known gene and disrupting its function to observe what happens
Stack of flattened membrane bags that packages and glycosylates protiens
A type of microscope that bombards samples with elections in a raster pattern to see the surface
Attaching antibodies to beads and letting it bind with a protein and associated moelcules
Focuses light on a specimen
The creation of two sets of DNA, happens before mitosis
The growth, synthesis, mitosis and cytokinesis of a cell
Physical exchange of regions of genous between homologues
DefinitionVocab Word
Division of a cell into two diploid daughter cells
Organelle that contains enzymes for fat oxidations and phosphorylation of ADP into ATP
A method that seperates components by running them in lanes
A seperation method that uses charged groups attached to beads
Mus musculus - used to study vertebrate functions
Flattened bags of membrane without ribosomes involved in lipid synthesis and deals with drugs and calcium
Proteins that can couple the downhill movement of one molecular to the uphill movement of another through the plasma membrane
Membrane vesicles that deliver ingested materials to lysosomes
A type of microscope that bombards samples with electrons that go through the sample
Intranuclear site of ribosomal RNA synthesis and assembly
Ways to improve the detection of a microscope beyond its limits
A vast database of genes and proteins that allows for better manipulation
Coating with metal to produce contrast
A method where specimens are dried from aqueous solutions of metal salts
 
The reading of mRNA into protien structures in ribosomes
Transfer of proteins from gel to a sheet of nylon
Specified signals incorporated into structures of proetiens and nucleic acids that route these moelcules to right place
The creation of secondary and tertiary structures in proteins to maximize attractions and to shield from water
Large organelle made of microtubules that primarily provides movement for cell through dynein movement
A methodology that assumes that it is possible to understand a system by breaking down and studying the parts
Recieve and process inputs for a variety of stimuli
Composed of microtubules and filaments, provides structure for the cell
Move things, includes dynein, kinesin and myosin
Consists of nuclear DNA compacted by associated protein
A cell or group of cells that only have half the normal number of chromosomes
A cell or group of cells that have the full complement of chromosomes
Simple bacterial organism used to study cellular hardware
Seperates components based on the size of the molecules
Membrane bound bags containing catalase and various oxidases
A circular DNA that self replicates in cytoplasm of a bacterium
A method that fragments proteins then measures by weight
Saccharomyces Cerevisiae - used to study basic cell fundtions
Breaking up a cell to release molecules through physical means
an example is mitochondrion, which was incorporated by an archaea billions of years ago to form eukaryotes
DefinitionVocab Word
A change in the DNA sequence
Display of thousands of tiny spots on a glass slide each different
A microscope that uses visible light to see
Complexes of protein and RNA that synthesize proteins
A PCR method that can create a mutation in the cloned DNA
One of the domains of biology, incorporated a bacteria billions of years ago to form eukaryotes
A machine that spins really fast
Creates contrast by interferance between light from the sample and a reference beam
Looks as if shaft of light coming at sample from an angle, produced by beam interferance
Insertion of a segment of DNA into a plasmid vector
Membrane bounded compartment containing the chromosomes etc
A thin, cylindrical projection of plasma membrane supported by actin filaments
Uses a sharp point of laser light that sweeps across a sample
Impermeable membrane bound bags of hydrolytic enzymes
Carries information for constructing a particular protein
Usually double stranded molecule composed of phosphate, sugar, base
A short cylinder of nine microtubule triplets in center of cell, creates microtubules
Arabidopsis Thaliana - used to study plant biology
Proteins that open selectively and facilitate passive movement across membrane
An annular junction sealing the gap between two epithelial cells
S. pombe - used to study basic cell functions
A detergent that dissociate components for gel electrophoresis
Pathway involving the amplification and transmission of signals
Insertion of RNA to decrease amount of protein
In this method, a ligand that binds a molecule is attached to a solid and purified
Dense network of intermediate filaments inside nucleus of eukaryotic cell
Complex cell with a nucleus and membrane bounded organelles
Web of materials outside cell that provide support and binding to other cells
Membrane bound pit on surface of cell that can receieve materials
Formed from arrangement of microtubules during division, pulls cellular components to either side
A pair of concentric membranes connected to ER, surrounds the nucleus
A preparation method that makes samples cold and then cracks them
cDNA copies of expressed genes

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