MCDB 205 - Cell Components and Research

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DefinitionVocab Word
Flattened bags of membrane without ribosomes involved in lipid synthesis and deals with drugs and calcium
A change in the DNA sequence
An annular junction sealing the gap between two epithelial cells
Membrane bounded compartment containing the chromosomes etc
Complexes of protein and RNA that synthesize proteins
A detergent that dissociate components for gel electrophoresis
A PCR method that can create a mutation in the cloned DNA
A method that seperates components by interactions
Usually double stranded molecule composed of phosphate, sugar, base
Membrane bound pit on surface of cell that can receieve materials
A preparation method that makes samples cold and then cracks them
Dictyostelium Discoideum - used to study basic cell functions
One of the domains of biology, incorporated a bacteria billions of years ago to form eukaryotes
Small organelles formed from microtubules that extend outside the cell, provide sensory information and motion
A cell or group of cells that have the full complement of chromosomes
Composed of microtubules and filaments, provides structure for the cell
Dense network of intermediate filaments inside nucleus of eukaryotic cell
Breaking up a cell to release molecules through physical means
Large gated channels that control all traffic to the nucleus
Pathway involving the amplification and transmission of signals
S. pombe - used to study basic cell functions
Consists of nuclear DNA compacted by associated protein
A machine that spins really fast
Insertion of RNA to decrease amount of protein
A sequence of DNA or RNA that codes for a protein
Intranuclear site of ribosomal RNA synthesis and assembly
Impermeable membrane bound bags of hydrolytic enzymes
A methodology that assumes that it is possible to understand a system by breaking down and studying the parts
Ways to improve the detection of a microscope beyond its limits
Drosophila Melanogaster - used to study multicellular functions
Proteins that open selectively and facilitate passive movement across membrane
Arabidopsis Thaliana - used to study plant biology
Display of thousands of tiny spots on a glass slide each different
cDNA copies of expressed genes
Membrane bound bags containing catalase and various oxidases
DefinitionVocab Word
Fusions with this enzyme are used for chromatography because it binds well to glutathione
C. Elegans - used to study animal functions
The creation of two sets of DNA, happens before mitosis
A seperation method that uses charged groups attached to beads
Web of materials outside cell that provide support and binding to other cells
Attaching antibodies to beads and letting it bind with a protein and associated moelcules
Creates contrast by interferance between light from the sample and a reference beam
Membrane vesicles that deliver ingested materials to lysosomes
Focuses light on a specimen
Lipid Bilayer that surrounds the cell and has protiens and transmitters
A cell or group of cells that only have half the normal number of chromosomes
Recieve and process inputs for a variety of stimuli
A reaction to produce large amounts of DNA segments using restriction enzymes
The copying of DNA into mRNA strands in nucleus
Move things, includes dynein, kinesin and myosin
Simple bacterial organism used to study cellular hardware
In this method, a ligand that binds a molecule is attached to a solid and purified
Lens that collects and recombines beams of light
A method where the interaction of two proteins attached to polymerase allow synthesis of a protein
A circular DNA that self replicates in cytoplasm of a bacterium
 
A thin, cylindrical projection of plasma membrane supported by actin filaments
A microscope that uses visible light to see
The DNA partner of a strand of RNA
A method that seperates components by running them in lanes
Formed from arrangement of microtubules during division, pulls cellular components to either side
Specified signals incorporated into structures of proetiens and nucleic acids that route these moelcules to right place
Insertion of a segment of DNA into a plasmid vector
A vast database of genes and proteins that allows for better manipulation
Division of a cell into two diploid daughter cells
The formation of cellular components from molecules without aid of templates or enzymes
Mus musculus - used to study vertebrate functions
Organelle that contains enzymes for fat oxidations and phosphorylation of ADP into ATP
The reading of mRNA into protien structures in ribosomes
Usually single stranded molecule composed of phosphate, sugar, base
DefinitionVocab Word
A short cylinder of nine microtubule triplets in center of cell, creates microtubules
Saccharomyces Cerevisiae - used to study basic cell fundtions
Stack of flattened membrane bags that packages and glycosylates protiens
The set of all genes in an organism
A type of microscopy that uses the absorbance and emission of light
A method of microscopy that just shines light and captures visible light on a white background
Carries information for constructing a particular protein
Proteins that actively transmit materials through the plasma membrane
Transfer of proteins from gel to a sheet of nylon
'microfilaments,' tracks for myosin motors
Organisms that have had so much study done on them that they are easy to use
Complex cell with a nucleus and membrane bounded organelles
A type of method of preparation that embeds cells in plastic and then slices it
Polypeptide chain of amino acids, many uses
Coating with metal to produce contrast
Uses a sharp point of laser light that sweeps across a sample
A protein that glows green and allows labling of cellular components
A method where specimens are dried from aqueous solutions of metal salts
Flattened bags of membrane with associated ribosomes that create integral proteins
A type of microscope that bombards samples with elections in a raster pattern to see the surface
A type of microscope that bombards samples with electrons that go through the sample
The growth, synthesis, mitosis and cytokinesis of a cell
A cylindrical polymer of tubulin that forms cilia, flagella and mitotic spindles, provides tracks for dynein and kinesin
Looks as if shaft of light coming at sample from an angle, produced by beam interferance
A method that fragments proteins then measures by weight
an example is mitochondrion, which was incorporated by an archaea billions of years ago to form eukaryotes
Physical exchange of regions of genous between homologues
Starting with a known gene and disrupting its function to observe what happens
A pair of concentric membranes connected to ER, surrounds the nucleus
Seperates components based on the size of the molecules
The creation of secondary and tertiary structures in proteins to maximize attractions and to shield from water
Large organelle made of microtubules that primarily provides movement for cell through dynein movement
Proteins that can couple the downhill movement of one molecular to the uphill movement of another through the plasma membrane

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