MCDB 205 - Cell Components and Research

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DefinitionVocab Word
Transfer of proteins from gel to a sheet of nylon
An annular junction sealing the gap between two epithelial cells
Division of a cell into two diploid daughter cells
Saccharomyces Cerevisiae - used to study basic cell fundtions
Membrane bound bags containing catalase and various oxidases
Creates contrast by interferance between light from the sample and a reference beam
A type of method of preparation that embeds cells in plastic and then slices it
A detergent that dissociate components for gel electrophoresis
A circular DNA that self replicates in cytoplasm of a bacterium
The growth, synthesis, mitosis and cytokinesis of a cell
A change in the DNA sequence
A short cylinder of nine microtubule triplets in center of cell, creates microtubules
The reading of mRNA into protien structures in ribosomes
C. Elegans - used to study animal functions
In this method, a ligand that binds a molecule is attached to a solid and purified
Seperates components based on the size of the molecules
A preparation method that makes samples cold and then cracks them
Move things, includes dynein, kinesin and myosin
Physical exchange of regions of genous between homologues
Membrane vesicles that deliver ingested materials to lysosomes
Usually double stranded molecule composed of phosphate, sugar, base
A sequence of DNA or RNA that codes for a protein
Mus musculus - used to study vertebrate functions
A seperation method that uses charged groups attached to beads
Proteins that actively transmit materials through the plasma membrane
A thin, cylindrical projection of plasma membrane supported by actin filaments
Formed from arrangement of microtubules during division, pulls cellular components to either side
Proteins that open selectively and facilitate passive movement across membrane
Looks as if shaft of light coming at sample from an angle, produced by beam interferance
Uses a sharp point of laser light that sweeps across a sample
Ways to improve the detection of a microscope beyond its limits
Impermeable membrane bound bags of hydrolytic enzymes
Pathway involving the amplification and transmission of signals
Arabidopsis Thaliana - used to study plant biology
Insertion of a segment of DNA into a plasmid vector
DefinitionVocab Word
Focuses light on a specimen
A cell or group of cells that only have half the normal number of chromosomes
A type of microscope that bombards samples with elections in a raster pattern to see the surface
Membrane bounded compartment containing the chromosomes etc
A machine that spins really fast
Large organelle made of microtubules that primarily provides movement for cell through dynein movement
A method that seperates components by running them in lanes
Starting with a known gene and disrupting its function to observe what happens
Flattened bags of membrane without ribosomes involved in lipid synthesis and deals with drugs and calcium
Usually single stranded molecule composed of phosphate, sugar, base
Lens that collects and recombines beams of light
A method of microscopy that just shines light and captures visible light on a white background
an example is mitochondrion, which was incorporated by an archaea billions of years ago to form eukaryotes
Proteins that can couple the downhill movement of one molecular to the uphill movement of another through the plasma membrane
A method that seperates components by interactions
Intranuclear site of ribosomal RNA synthesis and assembly
Drosophila Melanogaster - used to study multicellular functions
The copying of DNA into mRNA strands in nucleus
Composed of microtubules and filaments, provides structure for the cell
Membrane bound pit on surface of cell that can receieve materials
Small organelles formed from microtubules that extend outside the cell, provide sensory information and motion
Organisms that have had so much study done on them that they are easy to use
Flattened bags of membrane with associated ribosomes that create integral proteins
A methodology that assumes that it is possible to understand a system by breaking down and studying the parts
Dictyostelium Discoideum - used to study basic cell functions
The DNA partner of a strand of RNA
S. pombe - used to study basic cell functions
Display of thousands of tiny spots on a glass slide each different
A protein that glows green and allows labling of cellular components
The set of all genes in an organism
Fusions with this enzyme are used for chromatography because it binds well to glutathione
Large gated channels that control all traffic to the nucleus
Carries information for constructing a particular protein
Complex cell with a nucleus and membrane bounded organelles
A method that fragments proteins then measures by weight
DefinitionVocab Word
The creation of two sets of DNA, happens before mitosis
Insertion of RNA to decrease amount of protein
Lipid Bilayer that surrounds the cell and has protiens and transmitters
One of the domains of biology, incorporated a bacteria billions of years ago to form eukaryotes
A cell or group of cells that have the full complement of chromosomes
Simple bacterial organism used to study cellular hardware
The creation of secondary and tertiary structures in proteins to maximize attractions and to shield from water
'microfilaments,' tracks for myosin motors
Complexes of protein and RNA that synthesize proteins
A method where specimens are dried from aqueous solutions of metal salts
A pair of concentric membranes connected to ER, surrounds the nucleus
Specified signals incorporated into structures of proetiens and nucleic acids that route these moelcules to right place
A cylindrical polymer of tubulin that forms cilia, flagella and mitotic spindles, provides tracks for dynein and kinesin
A method where the interaction of two proteins attached to polymerase allow synthesis of a protein
A vast database of genes and proteins that allows for better manipulation
The formation of cellular components from molecules without aid of templates or enzymes
A PCR method that can create a mutation in the cloned DNA
Recieve and process inputs for a variety of stimuli
Breaking up a cell to release molecules through physical means
A microscope that uses visible light to see
Polypeptide chain of amino acids, many uses
A type of microscopy that uses the absorbance and emission of light
Stack of flattened membrane bags that packages and glycosylates protiens
A type of microscope that bombards samples with electrons that go through the sample
 
Dense network of intermediate filaments inside nucleus of eukaryotic cell
Organelle that contains enzymes for fat oxidations and phosphorylation of ADP into ATP
A reaction to produce large amounts of DNA segments using restriction enzymes
Attaching antibodies to beads and letting it bind with a protein and associated moelcules
Coating with metal to produce contrast
Consists of nuclear DNA compacted by associated protein
Web of materials outside cell that provide support and binding to other cells
cDNA copies of expressed genes

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