MCDB 205 - Cell Components and Research

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DefinitionVocab Word
Transfer of proteins from gel to a sheet of nylon
Starting with a known gene and disrupting its function to observe what happens
Membrane bounded compartment containing the chromosomes etc
A method that fragments proteins then measures by weight
Proteins that open selectively and facilitate passive movement across membrane
Fusions with this enzyme are used for chromatography because it binds well to glutathione
A seperation method that uses charged groups attached to beads
A change in the DNA sequence
Mus musculus - used to study vertebrate functions
Physical exchange of regions of genous between homologues
A cell or group of cells that have the full complement of chromosomes
A method that seperates components by running them in lanes
Seperates components based on the size of the molecules
Division of a cell into two diploid daughter cells
A type of microscope that bombards samples with elections in a raster pattern to see the surface
Uses a sharp point of laser light that sweeps across a sample
A method that seperates components by interactions
Proteins that can couple the downhill movement of one molecular to the uphill movement of another through the plasma membrane
Ways to improve the detection of a microscope beyond its limits
The creation of secondary and tertiary structures in proteins to maximize attractions and to shield from water
Looks as if shaft of light coming at sample from an angle, produced by beam interferance
Coating with metal to produce contrast
A pair of concentric membranes connected to ER, surrounds the nucleus
Impermeable membrane bound bags of hydrolytic enzymes
One of the domains of biology, incorporated a bacteria billions of years ago to form eukaryotes
A microscope that uses visible light to see
Drosophila Melanogaster - used to study multicellular functions
Polypeptide chain of amino acids, many uses
A method where specimens are dried from aqueous solutions of metal salts
Organelle that contains enzymes for fat oxidations and phosphorylation of ADP into ATP
Large gated channels that control all traffic to the nucleus
A cylindrical polymer of tubulin that forms cilia, flagella and mitotic spindles, provides tracks for dynein and kinesin
Formed from arrangement of microtubules during division, pulls cellular components to either side
Intranuclear site of ribosomal RNA synthesis and assembly
The copying of DNA into mRNA strands in nucleus
DefinitionVocab Word
Dense network of intermediate filaments inside nucleus of eukaryotic cell
Pathway involving the amplification and transmission of signals
Usually double stranded molecule composed of phosphate, sugar, base
Membrane bound pit on surface of cell that can receieve materials
Proteins that actively transmit materials through the plasma membrane
A method where the interaction of two proteins attached to polymerase allow synthesis of a protein
Composed of microtubules and filaments, provides structure for the cell
Insertion of RNA to decrease amount of protein
A thin, cylindrical projection of plasma membrane supported by actin filaments
an example is mitochondrion, which was incorporated by an archaea billions of years ago to form eukaryotes
Move things, includes dynein, kinesin and myosin
A PCR method that can create a mutation in the cloned DNA
The creation of two sets of DNA, happens before mitosis
A reaction to produce large amounts of DNA segments using restriction enzymes
The reading of mRNA into protien structures in ribosomes
S. pombe - used to study basic cell functions
Complex cell with a nucleus and membrane bounded organelles
A type of microscope that bombards samples with electrons that go through the sample
Consists of nuclear DNA compacted by associated protein
Web of materials outside cell that provide support and binding to other cells
Large organelle made of microtubules that primarily provides movement for cell through dynein movement
Membrane bound bags containing catalase and various oxidases
A sequence of DNA or RNA that codes for a protein
A machine that spins really fast
A methodology that assumes that it is possible to understand a system by breaking down and studying the parts
'microfilaments,' tracks for myosin motors
A short cylinder of nine microtubule triplets in center of cell, creates microtubules
Recieve and process inputs for a variety of stimuli
cDNA copies of expressed genes
The formation of cellular components from molecules without aid of templates or enzymes
Insertion of a segment of DNA into a plasmid vector
Flattened bags of membrane with associated ribosomes that create integral proteins
The DNA partner of a strand of RNA
Stack of flattened membrane bags that packages and glycosylates protiens
Carries information for constructing a particular protein
DefinitionVocab Word
A protein that glows green and allows labling of cellular components
Usually single stranded molecule composed of phosphate, sugar, base
Membrane vesicles that deliver ingested materials to lysosomes
The set of all genes in an organism
C. Elegans - used to study animal functions
A type of microscopy that uses the absorbance and emission of light
Complexes of protein and RNA that synthesize proteins
A detergent that dissociate components for gel electrophoresis
Arabidopsis Thaliana - used to study plant biology
A vast database of genes and proteins that allows for better manipulation
A preparation method that makes samples cold and then cracks them
An annular junction sealing the gap between two epithelial cells
Display of thousands of tiny spots on a glass slide each different
A circular DNA that self replicates in cytoplasm of a bacterium
In this method, a ligand that binds a molecule is attached to a solid and purified
A method of microscopy that just shines light and captures visible light on a white background
Lens that collects and recombines beams of light
Lipid Bilayer that surrounds the cell and has protiens and transmitters
Saccharomyces Cerevisiae - used to study basic cell fundtions
Breaking up a cell to release molecules through physical means
Focuses light on a specimen
The growth, synthesis, mitosis and cytokinesis of a cell
Flattened bags of membrane without ribosomes involved in lipid synthesis and deals with drugs and calcium
A cell or group of cells that only have half the normal number of chromosomes
Specified signals incorporated into structures of proetiens and nucleic acids that route these moelcules to right place
A type of method of preparation that embeds cells in plastic and then slices it
Dictyostelium Discoideum - used to study basic cell functions
Simple bacterial organism used to study cellular hardware
Attaching antibodies to beads and letting it bind with a protein and associated moelcules
 
Organisms that have had so much study done on them that they are easy to use
Small organelles formed from microtubules that extend outside the cell, provide sensory information and motion
Creates contrast by interferance between light from the sample and a reference beam

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